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In bone marrow core biopsy, in-tact bone marrow is obtained for laboratory analysis. These observations suggestedthe oncogenic consequences of mdm2 gene ampli? cation [ 1], and were followed bythe ?ndings that the mdm2 gene is ampli?ed and overexpressed in 17 of 47 sarco-mas, and 5 out of 5 sarcomas with mdm2 gene ampli?cation did not have a p53 genemutation commonly found in human tumors [ 2 ]

These observations suggestedthe oncogenic consequences of mdm2 gene ampli? cation [ 1], and were followed bythe ?ndings that the mdm2 gene is ampli?ed and overexpressed in 17 of 47 sarco-mas, and 5 out of 5 sarcomas with mdm2 gene ampli?cation did not have a p53 genemutation commonly found in human tumors [ 2 ].

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Similar histochemical proce-dures have been developed to demonstrate alkaline phospha-tase, adenosine triphosphatases (ATPases) of many varieties(including the Na+/K+ATPase that is the enzymatic basis ofthe sodium pump in cells and tissues), various esterases, andmany respiratory enzymes (Fig. One-stage exchange arthroplasty for chronic peri-prosthetic hip infection: results of a large prospective cohort study.

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